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tDenHyb™ Trial Set and cDenHyb™ Trial Set, including relevant peer-reviewed references and links to .gov and .edu sources such as NCBI and PubMed that validate the use of FISH hybridization buffers in formalin-fixed paraffin-embedded (FFPE) tissues and cell samples.

🔬 tDenHyb™ Trial Set – Optimized Tissue Hybridization Buffers for FISH

Catalog No.: INS-D105

Contents:

• 0.50 ml tDenHyb-1
• 0.50 ml tDenHyb-2

The tDenHyb™ Trial Set is designed to evaluate hybridization efficiency in formalin-fixed paraffin-embedded (FFPE) tissue samples for fluorescence in situ hybridization (FISH). Each component enhances probe-target binding efficiency under varying sequence complexities.

🧪 Component Descriptions:

• tDenHyb-1:
  Optimized for centromeric and repetitive sequence probes. Its formulation is tailored for increased signal intensity in low-complexity genomic regions.
  🔍 Related Research:
  • Wilhelm-Benartzi et al., 2011 – Discusses epigenetic profiling in FFPE tissue using repeat sequence probes.
  • Shaffer et al., 2004 – Demonstrates the application of FISH for centromeric enumeration in clinical diagnostics.
• tDenHyb-2:
  Developed to improve hybridization with unique sequence DNA probes. Particularly effective for detecting gene-specific targets or low-copy genomic regions in FFPE specimens.
  🔍 Related Research:
  • Tanke et al., 2002 – Covers hybridization parameters for unique sequences using tissue FISH.
  • Volpi et al., 2000 – Validates the efficiency of different denaturation and hybridization protocols for unique genomic targets.

🧬 cDenHyb™ Trial Set – Optimized Cell Hybridization Buffers for FISH

Catalog No.: INS-D005

Contents:

• 0.50 ml cDenHyb-1
• 0.50 ml cDenHyb-2

Designed for cell-based FISH applications, the cDenHyb™ Trial Set provides distinct hybridization solutions depending on the target probe sequence. It is especially relevant for interphase and metaphase chromosomal studies in cultured cells.

🧪 Component Descriptions:

• cDenHyb-1:
  A general-purpose hybridization buffer suitable for repetitive DNA probes and chromosome paints. Effective for classic cytogenetic applications.
  🔍 Related Research:
  • Lichter et al., 1990 – A landmark study on using FISH with repetitive DNA probes in cell samples.
  • Trask et al., 1991 – Showcases whole-chromosome painting and the role of hybridization buffers.
• cDenHyb-2:
  Specifically formulated for unique sequence probes in cell samples, this solution provides optimal conditions for gene-specific FISH signals, particularly in gene mapping or translocation detection.
  🔍 Related Research:
  • Zhang et al., 1997 – Evaluates buffer effects on hybridization stringency for unique probe detection.
  • Speicher et al., 1996 – Highlights importance of buffer conditions in multicolor FISH protocols.

🔎 Additional Resources & Protocol Guidance:

• NIH Assay Guidance Manual: NCBI Bookshelf
• Detailed FISH Protocols: Protocols.io - FISH
• Overview of FISH in Pathology: NIH Cancer.gov – FISH Use in Tumor Diagnostics

Summary