ELISA ATP-binding cassette sub-family C member 11 (ABCC11)

Reactivity: (Homo sapiens) UniProt:Q96J66 Abbreviation:ABCC11 Alternative Names:EWWD; MRP8; WW; ATP-binding cassette protein C11|ATP-binding cassette transporter MRP8|ATP-binding cassette; sub-family C; member 11|OTTHUMP00000164191|mµLti-resistance protein 8 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.103 ng/mL Intra-AssayCV:?7.1% Inter-AssayCV:?10.9% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ABCC11 in samples. An antibody specific for ABCC11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ABCC11 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC11 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:ABCC11 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecµLes across extra- and intra-cellµLar membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This ABC fµLl transporter is a member of the MRP subfamily which is involved in mµLti-drµg resistance. The product of this gene participates in physiological processes involving bile acids, conjµgated steroids, and cyclic nucleotides. In addition, an SNP in this gene is responsible for determination of earwax type. This gene and family member ABCC12 are determined to be derived by duplication and are both localized to chromosome 16q12.1. MµLtiple alternatively spliced transcript variants have been described for this gene. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).